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Introduction
The VEGF Phospho Antibody
Array features 185 highly specific and
well-characterized antibodies. All phospho antibodies and
non-phospho antibodies on the array are
site-specific. This design allows
researchers to study proteins based on the
phosphorylation status. Each antibody is
printed with six replicates and covalently
immobilized on polymer coated glass slides.
The array slides can be scanned on all
microarray scanners that are compatible with
76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.
Target Protein
AKT(Ser473), AKT(Thr308), AKT(Tyr326),
AKT1(Thr450), AKT1(Thr72), AKT2(Ser474),
BAD(Ser112), BAD(Ser134), BAD(Ser136),
BAD(Ser155), BAD(Ser91/128), Casp9(Ser144),
Casp9(Ser196), Casp9(Tyr153),
c-PLA2(Ser505), Elk1(Ser383), Elk1(Ser389),
Elk1(Thr417), eNOS(Ser1177), eNOS(Ser615),
eNOS(Thr495), ERK1/2, ERK3(Ser189),
ERK8(Thr175/Tyr177), FAK(Ser910),
FAK(Tyr397), FAK(Tyr407), FAK(Tyr576),
FAK(Tyr861), FAK(Tyr925), FKHR(Ser256),
FKHR(Ser319), GRB2(Ser159), GTPase
activating protein (Phopspho-Ser387),
HSP27(Ser15), HSP27(Ser78), HSP27(Ser82),
HSP90 co-chaperone Cdc37(Ser13), HSP90A,
HSP90B(Ser254), IP3KA, IP3KC,
MAPKAPK2(Ser272), MAPKAPK2(Thr222),
MAPKAPK2(Thr334), MEK1(Ser217),
MEK1(Ser221), MEK1(Ser298), MEK1(Thr286),
MEK1(Thr291), MEK2(Thr394), MKK3(Ser189),
MKK3(Thr222), MKK6(Ser207), P38
MAPK(Thr180), P38 MAPK(Tyr182), P38
MAPK(Tyr322), p44/42 MAPK(Thr202), p44/42
MAPK(Tyr204), Paxillin(Tyr118),
Paxillin(Tyr31), PI3K-p85-a(Tyr607),
PI3K-p85-a/g(Tyr467/Tyr199), PKC-a(Tyr657),
PKC a/b II(Thr638), PKC-b(Ser661), PKC
delta(Ser645), PKC delta(Thr505), PKC
epsilon(Ser729), PKC theta(Ser676), PKC
theta(Thr538), PKC zeta (Ab-410), PKC zeta
(Ab-560), PKC zeta(Thr410), PKC
zeta(Thr560), PLC-g1(Tyr1253),
PLC-g1(Tyr771), PLC-g1(Tyr783),
PLC-g2(Tyr1217), PLC-g2(Tyr753),
Raf1(Phospho-Ser621), Raf1(Ser259),
Raf1(Ser338), Raf1(Tyr341), RapGEF1(Tyr504),
Ras-GRF1(Ser916), Shc(Tyr349), Shc(Tyr427),
Src(Ser75), Src(Tyr216), Src(Tyr418),
Src(Tyr529), VEGFR1(Tyr1333),
VEGFR2(Tyr1054), VEGFR2(Tyr1059),
VEGFR2(Tyr1175), VEGFR2(Tyr1214),
VEGFR2(Tyr951)
Complete Antibody List
How It Works
Key Features
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Unique
collection of site-specific phospho-specific
antibodies and non-phospho antibodies
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Microarray
format for high throughput expression
profiling protein extracts from cells,
frozen or FFPE tissues, or serum
samples
-
Each antibody
is spotted with six replicates for more
reliable and consistent results
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Antibodies
are covalently attached to 3D polymers on
glass substrates
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High
specificity and affinity; low background
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Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
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GenePix®
Array List (GAL) files are provided for
convenient and accurate data analysis
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Antibody Array Assay Kit
provides optimized reagents and buffers for
protein extraction, labeling, conjugation
and detection
Applications
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Protein
expression profiling (Qualitative)
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Measure
changes in phosphorylation status at
specific sites
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Compare
profiles of normal, diseased or treated
samples
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Identify
candidate biomarkers
Specifications
|
Slide
dimensions: |
76 x
25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x
9 mm |
Spot-to-spot distance: |
600
um |
Technical
Information
Antibody Array User's Guide
Antibody List / Reactivity
Array Layout
GAL File
Frequently
Asked Questions
Components
Antibody
arrays (2)
Storage Conditions
4°C for 6
months
Selected References
Zhang YM,
Dai BL, A novel
angiogenesis inhibitor impairs lovo cell
survival via targeting against human VEGFR
and its signaling pathway of phosphorylation.
Cell Death and Disease 2012, 3, e406;
doi:10.1038/cddis.2012.145
Additional
references
Ordering Information
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