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Introduction
The T-Cell
Receptor Signaling Phospho-Specific Antibody
Array features 188 highly specific and
well-characterized antibodies important to
T-cell signaling. All phospho antibodies and
non-phospho antibodies on the array are
site-specific. This design allows
researchers to study proteins based on the
phosphorylation status. Each antibody is
printed with six replicates and covalently
immobilized on polymer coated glass slides.
The array slides can be scanned on all
microarray scanners that are compatible with
76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.
Target Protein
AKT(Ser473), AKT(Thr308), AKT(Tyr326),
Calmodulin(Thr79/Ser81), CD28(Tyr218),
CD3Z(Tyr142), CD4(Ser433), CD45(Ser1007),
c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73),
c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93),
c-Jun(Tyr170), ERK1(Thr202), ERK1(Tyr204),
ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177),
Fos(Ser362), Fos(Thr232), FosB(Ser27),
GAD1/GAD2, GAD67/GAD1, GADD153,
GRB2(Ser159), IkB-a(Ser32/36),
IkB-a(Tyr305), IkB-a(Tyr42), IkB-b(Thr19),
IkB-e(Ser22), IKK-a(Thr23), IKK-a/b(Ser180/181),
IKK-b(Tyr188), IKK-b(Tyr199), IKK-g(Ser31),
IKK-g(Ser85), IP3KA, IP3KC,
JNK1/2/3(Thr183/Tyr185), LAT(Tyr171),
LAT(Tyr191), LAT3, LCK(Ser59), LCK(Tyr192),
LCK(Tyr393), LCK(Tyr504), MEK1(Ser217),
MEK1(Ser221), MEK1(Ser298), MEK1(Thr286),
MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4,
MKK3(Ser189), MKK3/MAP2K3(Thr222),
MKK6(Ser207), MKK7/MAP2K7(Ser271),
MKK7/MAP2K7(Thr275), NFAT3, NFAT4(Ser165),
NFkB-p100/p52(Ser865),
NFkB-p100/p52(Ser869), NFkB-p105(Ser927),
NFkB-p105/p50(Ser337),
NFkB-p105/p50(Ser932), NFkB-p65(Ser536),
NFkB-p65(Thr254), p38 MAPK(Thr180), p38
MAPK(Tyr182), p38 MAPK(Tyr322),
PI3K-p85-a(Tyr607),
PI3K-p85-a/g(Tyr467/199), PKC pan activation
sites, PKC theta(Ser676), PKC theta(Thr538),
Rac1/cdc42(Ser71), Raf1(Ser259),
Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341),
Ras-GRF1(Ser916), Rel(Ser503), RelB(Ser552),
SLP-76(Tyr128), VAV(Tyr174), VAV2(Tyr142),
WASP(Tyr290), ZAP-70(Tyr292),
ZAP-70(Tyr315), Zap-70(Tyr319),
Zap-70(Tyr493)
Complete Antibody List
How It Works
Key Features
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Unique
collection of site-specific phospho-specific antibodies
and non-phospho antibodies
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Microarray
format for high throughput expression
profiling protein extracts from cells, frozen or FFPE tissues, or serum samples
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Each antibody
is spotted with six replicates for more reliable
and consistent results
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Antibodies
are covalently attached to 3D polymers on glass substrates
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High
specificity and affinity; low background
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Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
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GenePix®
Array List (GAL) files are
provided for convenient and accurate data
analysis
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Antibody Array
Assay Kit
provides optimized reagents
and buffers for protein
extraction, labeling, conjugation and
detection
Applications
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Protein
expression profiling (Qualitative)
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Measure
changes in phosphorylation status at
specific sites
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Compare
profiles of normal, diseased or treated
samples
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Identify
candidate biomarkers
Specifications
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Slide
dimensions: |
76 x
25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x
9 mm |
Spot-to-spot distance: |
600
um |
Technical
Information
Antibody Array User's Guide
Antibody List / Reactivity
Array Layout
GAL File
Frequently
Asked Questions
Components
Antibody
arrays (2)
Storage Conditions
4°C for 6
months
Selected References
Ordering Information
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