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GPCR Signaling to MAPK/ERK Phospho Antibody Array

Introduction

Each G-Protein Coupled Receptors (GPCRs) Signaling to MAPK/ERK Phospho Antibody Array features 193 highly specific and well-characterized phosphorylation antibodies important to this signaling pathway.  The phospho-specific antibodies bind to and detect the protein only when phosphorylated at the specific site.  The non-phospho pairs of the phospho-specific antibodies are included in the array to allow comparison based on phosphorylation state.  Each antibody is printed with six replicates, and the arrays are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.   

Key Features

• Unique collection of phospho-specific antibodies and their non-phospho pairs

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

• Each antibody is spotted with six replicates for more reliable and consistent results

• 3-D polymer based glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at specific sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 - 280 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

Technical Information

Antibody Array User's Guide

Antibody List / Reactivity

Array Layout

GAL File

Frequently Asked Questions

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

Selected References

Ordering Information

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