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EGF Pathway Phospho Antibody Microarray

Introduction

The EGF Phospho Antibody Array features 137 highly specific and well-characterized antibodies related to the EGF pathway. All phospho antibodies and non-phospho antibodies on the array are site-specific. This design allows researchers to study proteins based on the phosphorylation status. Each antibody is printed with six replicates and covalently immobilized on polymer coated glass slides. The array slides can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.

Target Protein

AKT(Ser473), AKT(Thr308), AKT(Tyr326), Catenin beta(Ser33), Catenin beta(Ser37), Catenin beta(Thr41/Ser45), Catenin beta(Tyr489), Catenin beta(Tyr654), Caveolin-1(Tyr14), CBL(Tyr700), CBL(Tyr774), c-Raf(Ser296), c-Raf(Ser43), Dok-2(Tyr299), E-cadherin, EGFR(Ser1070), EGFR(Thr678), EGFR(Thr693), EGFR(Tyr1016), EGFR(Tyr1069), EGFR(Tyr1092), EGFR(Tyr1110), EGFR(Tyr1172), EGFR(Tyr1197), EGFR(Tyr869), EMR1, EMR2, EMR3, ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), FAK(Ser910), FAK(Tyr397), FAK(Tyr407), FAK(Tyr576), FAK(Tyr861), FAK(Tyr925), Gab1(Tyr627), Gab1(Tyr659), GRB2(Ser159), HER2(Tyr1221/Tyr1222), HER2(Tyr1248), HER2(Tyr877), IKK gamma(Ser85), IKK-alpha(Thr23), IKK-alpha/beta(Ser180/181), IKK-beta(Tyr188), IKK-beta(Tyr199), IKK-gamma(Ser31), IP3KA, IP3KC, IP6K2, IP6K3, JAK1(Tyr1022), JAK2(Tyr1007), JAK2(Tyr221), JNK1/2/3(Thr183/Tyr185), JNKK, MAP3K1/MEKK1(Thr1381), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), p44/42 MAPK(Thr202), p44/42 MAPK(Tyr204), PAK1(Ser204), PAK1(Thr212), PAK1/2(Ser199), PAK1/2/3(Thr423/402/421), Paxillin(Tyr118), Paxillin(Tyr31), PDK1(Ser241), PI3K-p85-alpha(Tyr607), PI3K-p85-alpha/gamma(Tyr467/Tyr199), PIP5K, PKC pan activation site, PLCG1(Tyr1253), PLCG1(Tyr771), PLCG1(Tyr783), PLCG2(Tyr1217), PLCG2(Tyr753), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), RAS (p21 H&K), RASE, RASF4, Ras-GRF1(Ser916), Rho/Rac GEF2(Ser885), SAPK/JNK(Thr183), SAPK/JNK(Tyr185), SEK1/MKK4(Ser80), SEK1/MKK4(Thr261), SEK1/MKK4/JNKK1 (Ser257), Shc(Tyr349), Shc(Tyr427), Src(Ser75), Src(Tyr216), Src(Tyr418), Src(Tyr529), STAT1(Ser727), STAT1(Tyr701), STAT3(Ser727), STAT3(Tyr705), VAV1(Tyr174), VAV2(Tyr142)

Complete Antibody List

How It Works

Key Features

• Unique collection of site-specific phospho-specific antibodies and non-phospho antibodies

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues,  or serum samples

• Each antibody is spotted with six replicates for more reliable and consistent results

• Antibodies are covalently attached to 3D polymers on glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at specific sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 - 280 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

Technical Information

Antibody Array User's Guide

Antibody List / Reactivity

Array Layout

GAL File

Frequently Asked Questions

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

Selected References

Ordering Information

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