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Introduction
The
Cytoskeleton Phospho Antibody Array consists of 141 well-characterized phosphorylation
antibodies in the cytoskeletal pathway. The phospho-specific
antibodies bind to and detect the protein
only when phosphorylated at the specific
site. The non-phospho pairs of the
phospho-specific antibodies are included in
the array to allow comparison based on
phosphorylation state. Each antibody
is printed with six replicates, and the
arrays are printed on standard-size
coated glass microscope
slides and can be scanned on all microarray
scanners that are compatible with 76 x 25 x 1 mm
(3 in. x 1 in. x 1mm) slides.
Target Protein
Actin Pan(a/b/g)(Tyr55/53), Actin alpha-2/3,
Actin-alpha1 sk muscle, ACTN1,
Calmodulin(Thr79/Ser81),
CaMK1-alpha(Thr177), CaMK1-beta,
CaMK2(Thr286), CaMK2(Thr305), CaMK2-b/g/d(Thr287),
CaMK4(Thr196/200), CAMK5, Cofilin(Ser3),
Cortactin(Tyr421), Cortactin(Tyr466),
c-Raf(Ser296), c-Raf(Ser43), CrkII(Tyr221),
CrkL(Tyr207), ERK1/2, ERK3(Ser189),
ERK8(Thr175/Tyr177), Ezrin(Thr566),
Ezrin(Tyr353), Ezrin(Tyr478), FAK(Ser910),
FAK(Tyr397), FAK(Tyr407), FAK(Tyr576),
FAK(Tyr861), FAK(Tyr925), Filamin
A(Ser2152), GRB2(Ser159), GTPase activating
protein(Ser387), LIMK1(Thr508),
MEK1(Ser217), MEK1(Ser221), MEK1(Thr291),
MEK1(Ser298), MEK1(Thr286), MEK2(Thr394),
MEKKK1, MEKKK4, Merlin(Ser10),
Merlin(Ser518), MKK3(Ser189),
MKK3/MAP2K3(Thr222), MKK6(Se207),
MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275),
Myosin regulatory light chain 2(Ser18),
NCK2, p130Cas(Tyr165), p130Cas(Tyr410),
p44/42 MAPK(Thr202), p44/42 MAPK(Tyr204),
Paxillin(Tyr118), Paxillin(Tyr31),
PI3K-p85-alpha(Tyr607), PI3K-p85-a/g(Tyr467/Tyr199),
PIP5K, PIP5K(Ser307), PKA CAT(Thr197), PKC
alpha (Tyr657), PKC alpha/beta II(Thr638),
PKC pan activation site, PLC beta(Ser1105),
PLC beta3(Ser537), Rac1/cdc42(Ser71), Rho/Rac
GEF(Ser885), Src(Ser75), Src(Tyr216),
Src(Tyr418), Src(Tyr529), VASP(Ser157),
VASP(Ser238), WASP(Tyr290), WAVE1(Tyr125)
Complete Antibody List
How It Works
Key
Features
-
Unique
collection of site-specific phospho-specific antibodies
and non-phospho antibodies
-
Microarray
format for high throughput expression
profiling protein extracts from cells, frozen or FFPE tissues, or serum samples
-
Each antibody
is spotted with six replicates for more reliable
and consistent results
-
Antibodies
are covalently attached to 3D polymers on glass substrates
-
High
specificity and affinity; low background
-
Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
-
GenePix®
Array List (GAL) files are
provided for convenient and accurate data
analysis
-
Antibody Array
Assay Kit
provides optimized reagents
and buffers for protein
extraction, labeling, conjugation and
detection
Applications
-
Protein expression
profiling (Qualitative)
-
Measure
changes in phosphorylation status at
specific sites
-
Compare
profiles of normal, diseased or treated
samples
-
Identify
candidate biomarkers
Specifications
|
Slide
dimensions: |
76 x 25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x 9 mm |
Spot-to-spot distance: |
600 um |
Technical
Information
Antibody Array User's Guide
Antibody List / Reactivity
Array Layout
Gal File
Frequently
Asked Questions
Components
Antibody
arrays (2)
Storage Conditions
4°C for 6 months
Selected References
El-Haibi CP,
Singh R, Antibody Microarray
Analysis of Signaling Networks Regulated by
Cxcl13 and Cxcr5 in Prostate Cancer.
Journal of Proteomics and Bioinformatics
2012, 5(8): 177-184
Gomez JC,
Doerschuk CM: Rac2 in
murine pulmonary microvascular endothelial
cells (ECs),
FASEB J. 24: 777.11
Additional
references
Ordering Information
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