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Cytoskeleton Phospho-Specific Antibody Microarray


Introduction

Each Cytoskeleton Phospho-Antibody Array features 95 highly specific and well-characterized phosphorylation antibodies in the cytoskeletal pathway; each has six replicates.  The phospho-specific antibodies bind to and detect the protein only when phosphorylated at the specific site.  The non-phospho pairs of the phospho-specific antibodies are included in the array to allow comparison based on phosphorylation state.  The antibodies are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.    


Key Features

  • Unique collection of phospho-specific antibodies and their non-phospho pairs

  • Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

  • Each antibody is spotted with six replicates for more reliable and consistent results

  • 3-D polymer based glass substrates

  • High specificity and affinity; low background

  • Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

  • GenePix® Array List (GAL) files are provided for convenient and accurate data analysis

  • Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection


Applications

  • Protein expression profiling (Qualitative)

  • Measure changes in phosphorylation status at specific sites

  • Compare profiles of normal, diseased or treated samples

  • Identify candidate biomarkers


Specifications

Slide dimensions:

76 x 25 x 1 mm

Spot diameter: 260 - 280 um
Barcode label: 23 x 9 mm Spot-to-spot distance: 600 um

Technical Information

Antibody Array User's Guide

Antibody List

Array Layout

Gal File

Frequently Asked Questions


Components

Antibody arrays (2)


Storage Conditions

4°C for 6 months


Selected References

Gomez JC, Doerschuk CM: Rac2 in murine pulmonary microvascular endothelial cells (ECs), FASEB J. 24: 777.11


Ordering Information

Cat. No.

Description

Reactivity

No. of Antibodies

Size

Price (US$)

PCP095

Cytoskeleton Phospho-Specific Antibody Array

Human

Mouse

95

2 slides

395

 


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KAS02

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KAS20

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SEV03

Complete Antibody Array Assay Service, Cytoskeleton Phospho Array (PCP095)

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