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Introduction
The AMPK
Phospho Antibody Array features 174 highly
specific and well-characterized antibodies
related to the AMPK pathway. All phospho
antibodies and non-phospho antibodies on the
array are site-specific. This design allows
researchers to study proteins based on the
phosphorylation status. Each antibody is
printed with six replicates and covalently
immobilized on polymer coated glass slides.
The array slides can be scanned on all
microarray scanners that are compatible with
76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.
Target Protein
4E-BP1(Ser65), 4E-BP1(Thr36), 4E-BP1(Thr45),
4E-BP1(Thr70), ACC1(Ser79), ACC1(Ser80),
AKT(Ser473), AKT(Thr308), AKT(Tyr326),
AKT1(Ser124), AKT1(Ser246), AKT1(Thr450),
AKT1(Thr72), AKT1(Tyr474), AKT1/2/3,
AKT1S1(Thr246), AKT2(Ser474), AMPK1(Thr174),
AMPK1/AMPK2(Ser485/491), AMPKbeta1(Ser182),
CaMK1-alpha(Thr177), CaMK1-beta,
CaMK2(Thr305), CaMK2(Thr286),
CaMK2-b/g/d(Thr287),
CaMK4(Thr196/200), CAMK5, Cyclin A1, Cyclin
B1(Ser126), Cyclin B1(Ser147), EEF2(Thr56),
eEF2K(Ser366), eNOS(Ser1177), eNOS(Ser615),
eNOS(Thr495), Fas ligand, HMG14, HMG20B,
HMGB1, HMGB2, HNF4a(Ser304), HNF4a/g,
HSF1(Ser303), HSL(Ser552/563), HSL(Ser554),
LKB1(Ser428), LKB1(Thr189), MEF2A(Ser408),
MEF2A(Thr312), MEF2A(Thr319), MEF2B,
MEF2C(Ser396), MEF2D(Ser444), mTOR(Ser2448),
mTOR(Ser2481), mTOR(Thr2446),
p21Cip1(Thr145), p53(Ser6), p53(Ser9),
p53(Ser15), p53(Ser20), p53(Ser33),
p53(Ser37), p53(Ser46), p53(Ser315),
p53(Ser366), p53(Ser378), p53(Ser392),
p53(Thr18), p53(Thr81), p70S6K(Ser371),
p70S6K(Ser411), p70S6K(Ser418),
p70S6K(Ser424), p70S6K(Thr229),
p70S6K(Thr389), p70S6k(Thr421),
p70S6K-beta(Ser423), PFKFB2(Ser483), PI3K-p85-alpha(Tyr607), PI3K-p85-a/g(Tyr467/199), PKA
CAT(Thr197), PKA-R2B(Ser113),
PLC-beta(Ser1105), PLC-beta3(Ser537),
RapGEF1(Tyr504), SREBP-1(Ser439), Tuberin(Ser939),
Tuberin(Thr1462)
Complete Antibody List
How It Works
Key Features
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Unique
collection of site-specific phospho-specific antibodies
and non-phospho antibodies
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Microarray
format for high throughput expression
profiling protein extracts from cells, frozen or FFPE tissues, or serum samples
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Each antibody
is spotted with six replicates for more reliable
and consistent results
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Antibodies
are covalently attached to 3D polymers on glass substrates
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High
specificity and affinity; low background
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Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
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GenePix®
Array List (GAL) files are
provided for convenient and accurate data
analysis
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Antibody Array
Assay Kit
provides optimized reagents
and buffers for protein
extraction, labeling, conjugation and
detection
Applications
-
Protein
expression profiling (Qualitative)
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Measure
changes in phosphorylation status at
specific sites
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Compare
profiles of normal, diseased or treated
samples
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Identify
candidate biomarkers
Specifications
|
Slide
dimensions: |
76 x
25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x
9 mm |
Spot-to-spot distance: |
600
um |
Technical
Information
Antibody Array User's Guide
Antibody List / Reactivity
Array Layout
GAL File
Frequently
Asked Questions
Components
Antibody
arrays (2 slides)
Storage Conditions
4°C for 6
months
Selected References
Chen O,
Burke, JB, The chemokine
CCL5 regulates glucose uptake and AMPK
signaling in activated T cells to facilitate
chemotaxis.
Journal of Biological Chemistry 2012, 287:29406-29416
Additional
references
Ordering Information
Related
Service
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