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Apoptosis Antibody Microarray

Introduction

Each Apoptosis Antibody Array features 73 highly specific and well-characterized antibodies in the apoptosis pathway.  The arrays are printed on polymer coated glass slides and can be read on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.  Each antibody is printed with six replicates.

Target Protein

14.3.3 Pan, Acinus, AIF, Alpha-1-antichymotrypsin, alpha-1-antitrypsin, ARC, Ask1, ATM, BAG-1, Bak, Bax, Bcl10, bcl-2a, Bcl-6, bcl-X, bcl-XL, Bim, Caspase 1, Caspase 2, Caspase 3, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Cathepsin D, CD137, CD14, CD95, CDK2, CDK4, CDK5, c-fos, CIDE-A, CIDE-B, c-jun, CREB, CREB-Binding Protein, Cytochrome c, D4-GDI, Daxx, DcR1, DcR2, DFF40, DFF45, DR3, DR5, ERK1, ERK2, FADD, Fas-ligand, FLIP, Granzyme B, hsp70, I-FLICE, IGF-1R, IGF-I, IKKb, JKK1, MADD, Mcl-1, Mekk-1, NFkB/p50, NFkB/p65, p53, PARP, PARP, Prohibitin, RAIDD, SODD, Survivin, TNF alpha, TNFa, TRADD

Complete Antibody List

How It Works

Key Features

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

• Each antibody is spotted with six replicates for more reliable and consistent results

• Antibodies are covalently attached to 3D polymers on glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

Applications

• Protein expression profiling (Qualitative)

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 - 280 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

Technical Information

Antibody Array User's Guide

Antibody List / Reactivity

Array Layout

GAL File

Frequently Asked Questions           

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

Components

Molnar E: Applications of Protein and Small Molecule Microarrays, Acta Biologica Szegediensis 2008, 52(2): 333-356

Additional References

Ordering Information

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