Full Moon BioSystems, Inc.
   

Products Services Support Publications Company Contact Us

Arrays

Apoptosis
Cancer BioMarker
Cell Cycle
Cytokine Profiling
Cytokines
Explorer
Kinase
Signal Transduction
Signaling Explorer Array
Phospho AKT
Phospho AKT/PKB
Phospho AMPK
Phospho Apoptosis
Phospho CancerApoptosis
Phospho Cancer Signaling
Phospho Cell Cycle
Phospho Cell Cycle Control
Phospho Chromatin
Phospho CREB
Phospho Cytoskeleton
Phospho EGF
Phospho ErbB/Her
Phospho ERK
Phospho Explorer
Phospho FGF
Phospho GPCR
Phospho IGF-1R
Phospho Insulin Receptor
Phospho Jak/Stat
Phospho Jak/Stat II
Phospho MAPK
Phospho mTOR
Phospho Neuroscience
Phospho NF-kB
Phospho Nuclear Receptors
Phospho p53
Phospho PDGF
Phospho T-Cell Receptors
Phospho TGF-Beta
Phospho VEGF
Phospho TyrosineKinase
Phospho WNT Signaling
Tyrosine Phospho ProArray

 

Home > Products > Antibody Microarray

Antibody Microarray


Introduction

Antibody Array provides a high-throughput platform for efficient profiling of protein expressions.  Our comprehensive Phospho Explorer Array and Explorer Array allow investigators to examine hundreds of proteins in a single experiment, while the Pathway Antibody Arrays and Phosphorylation Antibody Arrays are designed for researchers to study highly relevant proteins in their specific research fields. 

The antibodies are covalently immobilized on high quality glass surface coated with our proprietary 3-D polymer materials to ensure high binding efficiency and specificity.  Each array includes well-characterized antibodies, positive controls and negative controls. To maximize data reliability, each antibody is printed with six replicates.  The arrays utilize fluorescent detection and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides. 


How It Works


Key Features

  • Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

  • Carefully selected, well-characterized antibodies

  • Each antibody is printed with replicates for reliable and consistent results

  • 3-D polymer based glass substrates

  • High specificity and affinity; low background

  • Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

  • GenePix® Array List (GAL) files are provided for convenient and accurate data analysis

  • Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, coupling and detection


Applications

  • Protein expression profiling (Qualitative)

  • Measure changes in phosphorylation status at specific sites (Phospho Antibody Arrays)

  • Compare profiles of normal, diseased or treated samples

  • Identify candidate biomarkers


Technical Information

Antibody Array User's Guide

Frequently Asked Questions


Array Selection

Exploratory Antibody Arrays

Signaling Explorer Antibody Array New

Explorer Antibody Array

Phospho Explorer Antibody Array

Tyrosine Phosphorylation ProArray

Phosphorylation Antibody Arrays

Pathway Antibody Arrays

 


Performance       

 

AKT Phospho-Specific Antibody Array — Lysates from control and treated H522 cells were labeled with biotin and detected with Cy3-Streptavidin. For proteins PTEN(Ser380) and c-Jun(Thr91), the phosphorylation change between the control sample and treated sample was 5.59 folds and 4.23 folds respectively.


Specifications

Slide dimensions:

76 x 25 x 1 mm

Spot diameter: 260 - 280 um
Barcode label: 23 x 9 mm Spot-to-spot distance: 600 um

Components

Antibody arrays, 2 slides/pack (suitable for analyzing two samples)


Storage Conditions

4°C for 6 months


Selected References

Eke I, Schneider L, EGFR/JIP-4/JNK2 Signaling Attenuates Cetuximab-Mediated Radiosensitization of Squamous Cell Carcinoma Cells, Cancer Research, 2012 73(1): 297-306

He H, Zong Y, et al. Sensing the Insulin Signaling Pathway with an Antibody Array, Proteomics Clinical Applications 2009;3:1440–1450.

Kung S, Elf S, et al. p90 Ribosomal S6 Kinase 2 Promotes Invasion and Metastasis of Human Head and Neck Squamous Cell Carcinoma Cells, Journal of Clinical Investigation 2010; 120(4):1165-1177.

Yalcin A, Clem B, et al. Nuclear Targeting of 6-Phosphofructo-2-Kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases, Journal of Biological Chemistry, 2009; 284:24223-24232.

Yu Y, Richardson DR, Cellular iron depletion stimulates the JNK and p38 MAPK signaling transduction pathways, dissociation of ASK1-thioredoxin, and activation of ASK1, Journal of Biological Chemistry 2011, 286(17):15413-27

Zhang YM, Dai BL, A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death and Disease 2012, 3, e406; doi:10.1038/cddis.2012.145

Zhong D, Liu X, et al. LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 2008; 68:7270-7277.

Zhong D, Li X, et al. The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathway Through IGF1R, Journal of Biological Chemistry 2009; 284:23225-23233.

Additional publications


Related Products and Services

Antibody Array Assay Kit

Protein Extraction Kit

Complete Antibody Array Assay Service

Antibody Array Scanning and Image Analysis Service

 

 

Copyright © 2013 Full Moon BioSystems, Inc. All rights reserved.