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Antibody
Microarray
Introduction
Antibody Array provides a
high-throughput platform for efficient profiling
of protein expressions. Our comprehensive
Phospho Explorer
Array and
Explorer
Array allow investigators to
examine hundreds of proteins
in a single experiment, while the
Pathway
Antibody Arrays
and
Phosphorylation
Antibody Arrays
are designed for
researchers to study highly relevant proteins in
their specific research fields.
The antibodies are covalently immobilized on
high quality glass
surface coated with our proprietary 3-D polymer
materials to ensure high binding efficiency and
specificity. Each
array includes well-characterized
antibodies, positive controls and negative
controls. To maximize data reliability, each
antibody is printed with six
replicates. The arrays utilize fluorescent
detection and can be scanned on all microarray
scanners that are compatible with 76 x 25 x 1 mm
(3 in. x 1 in. x 1mm) slides.
By utilizing our
Complete Antibody
Array Assay Service, investigators have the
option of sending test samples to experienced
scientists at Full Moon BioSystems who will
perform antibody array assays in a reliable and
efficient manner.
Exploratory
Antibody Arrays
Kinase Antibody ArrayNew
Phospho Explorer Antibody
Array
Phospho Explorer S1 Antibody Array
New
Explorer Antibody Array
Tyrosine Phosphorylation
ProArray
Phosphorylation
Antibody Arrays
Pathway Antibody Arrays
Key Features
-
 Microarray
format for high throughput expression
profiling protein extracts from cells,
frozen or FFPE tissues, or serum samples
-
Carefully selected,
well-characterized antibodies
-
Each antibody
is printed with replicates for reliable
and consistent results
-
3-D polymer
based glass substrates
-
High
specificity and affinity; low background
-
Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
-
GenePix®
Array List (GAL) files are provided for
convenient and accurate data analysis
-
Antibody Array Assay Kit
provides optimized reagents and buffers for
protein extraction, labeling, coupling and
detection
Applications
-
Protein
expression profiling (Qualitative)
-
Measure
changes in phosphorylation status at
specific sites (Phospho Antibody Arrays)
-
Compare
profiles of normal, diseased or treated
samples
-
Identify
candidate biomarkers
Performance
AKT Phospho-Specific Antibody Array — Lysates
from control and treated H522 cells were labeled
with biotin and detected with Cy3-Streptavidin.
For proteins PTEN(Ser380) and c-Jun(Thr91), the
phosphorylation change between the control
sample and treated sample was 5.59 folds and
4.23 folds respectively.
Specifications
|
Slide
dimensions: |
76 x
25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x
9 mm |
Spot-to-spot distance: |
600
um |
Technical
Information
Antibody Array User's Guide
Frequently
Asked Questions
Components
Antibody
arrays (2 slides)
Storage Conditions
4°C for 6
months
Selected References
He H, et al:
Sensing the Insulin Signaling Pathway with an
Antibody Array,
Proteomics Clinical Applications 2009, 3,
1440–1450
Yalcin A, Clem B, et al.:
Nuclear
Targeting of 6-Phosphofructo-2-Kinase
(PFKFB3)
Increases Proliferation via Cyclin-dependent
Kinases,
Journal of Biological Chemistry, 2009 284:
24223-24232
Molnar E:
Applications of Protein and Small Molecule
Microarrays, Acta Biologica
Szegediensis 2008, 52(2): 333-356
Raharijaona M, Le Pennec S, et al. (2009)
PGC-1-Related Coactivator Modulates
Mitochondrial-Nuclear Crosstalk through
Endogenous Nitric Oxide in a Cellular Model of
Oncocytic Thyroid Tumours. PLoS ONE 4(11):
e7964. doi:10.1371/journal.pone.0007964
Zhong D,
Liu X, et al.:
LKB1 Is Necessary for Akt-Mediated
Phosphorylation of Proapoptotic Proteins,
Cancer Research
68, 7270-7277, September 15, 2008
Zhong D,
Li X, et al.:
The Glycolytic
Inhibitor 2-Deoxyglucose Activates Multiple
Prosurvival Pathway Through IGF1R,
Journal of Biological Chemistry 2009 284:
23225-23233
Related Products and
Services
Antibody
Array Assay Kit
Protein Extraction Kit
Complete Antibody
Array Assay Service
Antibody
Array Scanning Service (free of charge)
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