Antibody Microarray provides a high-throughput platform for efficient profiling of protein expressions.  Our comprehensive Phospho Explorer Array and Explorer Array allows investigators to examine samples against hundreds of antibodies in a single experiment, while the Pathway Antibody Arrays and Phosphorylation Antibody Arrays are designed for researchers to study highly relevant proteins in their specific research fields.

The antibodies are covalently immobilized on high quality glass surface coated with our proprietary 3-D polymer materials to ensure high binding efficiency and specificity.  Each array includes well-characterized antibodies, positive controls and negative controls. To maximize data reliability, each antibody is printed with six replicates.  The arrays utilize fluorescent detection and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides. 

Also available: Antibody Array Assay Service

Exploratory Antibody Arrays

Phospho Explorer Antibody Array ^New

Explorer Antibody Array

Tyrosine Phosphorylation ProArray ^New

Phosphorylation Antibody Arrays

AKT Pathway

AKT/PKB Signaling

AMPK Signaling

Apoptosis

Cancer/Apoptosis

Cell Cycle

Cell Cycle Control

Chromatin/Transcription

CREB

Cytoskeleton

Cytoskeleton II

EGF Pathway

ErbB/HER Signaling

ERK Signaling

FGF Pathway

GPCR Signaling to MAPK/ERK

Insulin/Glucose

Insulin Receptor Pathway

IGF-1R Signaling

JAK/STAT

Jak/Stat II

MAPK Pathway

mTOR Signaling

Neuroscience

NF-kB Pathway

NF-kB II Signaling

Nuclear Receptors

p53 Signaling

PDGF

T-Cell Receptor Signaling

TGF-beta Signaling

Tyrosine Kinase Adaptors

VEGF Pathway

Pathway Antibody Arrays

Angiogenesis

Apoptosis

Cancer Markers

Cell Cycle

Cytokines

Hematopoiesis

Hormones

Signal Transduction

Stem Cell

Key Features

Microarray format for high throughput expression profiling

Suitable samples: cells, frozen or FFPE tissues, or serum samples

Carefully selected, well-characterized antibodies

3-D polymer based glass substrates

High specificity and affinity; low background

Excellent uniformity and spot morphology

Each antibody is printed with replicates for more reliable and consistent results

Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

Antibody Array Assay Kit provides optimized reagents and buffers for processing the arrays

Applications

Protein Expression Profiling (Qualitative)

Compare profiles of normal, diseased or treated samples

Identify Candidate Biomarkers

• Protein Expression Profiling (Qualitative)

Performance

AKT Phospho-Specific Antibody Array — Lysates from control and treated H522 cells were labeled with biotin and detected with Cy3-Streptavidin. For proteins PTEN(Ser380) and c-Jun(Thr91), the phosphorylation change between the control sample and treated sample was 5.59 folds and 4.23 folds respectively. 

Components

Antibody Arrays (2 Slides)

Technical Information

Antibody Array User's Guide

Frequently Asked Questions

References

Yalcin A, Clem B, et al.: Nuclear Targeting of 6-Phosphofructo-2-Kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases, Journal of Biological Chemistry, 2009. Published on May 27, 2009 as Manuscript, available at: http://www.jbc.org/cgi/doi/10.1074/jbc.M109.016816

Zhong D, Liu X, et al.: LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 2008, 68: 7270-7277.

Zhong D, Li X, et al.: The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathway Through IGF1R, Journal of Biological Chemistry, 2009. Published on May 27, 2009 as Manuscript, available at: http://www.jbc.org/cgi/doi/10.1074/jbc.M109.005280

Molnar E: Applications of Protein and Small Molecule Microarrays, Acta Biologica Szegediensis 2008, 52(2): 333-356.

Specifications

Storage Conditions

4°C for six months

Related Products and Services

Antibody Array Assay Kit

Protein Extraction Kit

Antibody Array Assay Service