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Antibody
Microarray
Introduction
Antibody Array provides a
high-throughput platform for efficient profiling
of protein expressions. Our comprehensive
Phospho Explorer
Array and
Explorer
Array allow investigators to
examine hundreds of proteins
in a single experiment, while the
Pathway
Antibody Arrays
and
Phosphorylation
Antibody Arrays
are designed for
researchers to study highly relevant proteins in
their specific research fields.
The antibodies are covalently immobilized on
high quality glass
surface coated with our proprietary 3-D polymer
materials to ensure high binding efficiency and
specificity. Each
array includes well-characterized
antibodies, positive controls and negative
controls. To maximize data reliability, each
antibody is printed with six
replicates. The arrays utilize fluorescent
detection and can be scanned on all microarray
scanners that are compatible with 76 x 25 x 1 mm
(3 in. x 1 in. x 1mm) slides.
How It Works
Key Features
-
 Microarray
format for high throughput expression
profiling protein extracts from cells,
frozen or FFPE tissues, or serum samples
-
Carefully selected,
well-characterized antibodies
-
Each antibody
is printed with replicates for reliable
and consistent results
-
3-D polymer
based glass substrates
-
High
specificity and affinity; low background
-
Suitable for
detection on all fluorescent microarray
scanners compatible with 76x25x1mm slides
-
GenePix®
Array List (GAL) files are provided for
convenient and accurate data analysis
-
Antibody Array Assay Kit
provides optimized reagents and buffers for
protein extraction, labeling, coupling and
detection
Applications
-
Protein
expression profiling (Qualitative)
-
Measure
changes in phosphorylation status at
specific sites (Phospho Antibody Arrays)
-
Compare
profiles of normal, diseased or treated
samples
-
Identify
candidate biomarkers
Technical
Information
Antibody Array User's Guide
Frequently
Asked Questions
Array Selection
Exploratory
Antibody Arrays
Signaling Explorer Antibody
Array New
Explorer Antibody Array
Phospho Explorer Antibody
Array
Tyrosine
Phosphorylation ProArray
Phosphorylation
Antibody Arrays
Pathway Antibody Arrays
Performance
AKT Phospho-Specific Antibody Array — Lysates
from control and treated H522 cells were labeled
with biotin and detected with Cy3-Streptavidin.
For proteins PTEN(Ser380) and c-Jun(Thr91), the
phosphorylation change between the control
sample and treated sample was 5.59 folds and
4.23 folds respectively.
Specifications
|
Slide
dimensions: |
76 x
25 x 1 mm |
Spot
diameter: |
260 -
280 um |
|
Barcode
label: |
23 x
9 mm |
Spot-to-spot distance: |
600
um |
Components
Antibody
arrays, 2 slides/pack (suitable for
analyzing two samples)
Storage Conditions
4°C for 6
months
Selected References
Eke I,
Schneider L,
EGFR/JIP-4/JNK2 Signaling Attenuates
Cetuximab-Mediated Radiosensitization of
Squamous Cell Carcinoma Cells,
Cancer Research, 2012 73(1):
297-306
He H, Zong
Y, et al.
Sensing the Insulin Signaling Pathway with an
Antibody Array,
Proteomics Clinical Applications 2009;3:1440–1450.
Kung S,
Elf S, et al.
p90 Ribosomal S6 Kinase 2 Promotes Invasion and
Metastasis of Human Head and Neck Squamous Cell
Carcinoma Cells, Journal of Clinical Investigation
2010; 120(4):1165-1177.
Yalcin A, Clem B,
et al.
Nuclear
Targeting of 6-Phosphofructo-2-Kinase
(PFKFB3)
Increases Proliferation via Cyclin-dependent
Kinases,
Journal of Biological Chemistry, 2009; 284:24223-24232.
Yu Y,
Richardson DR,
Cellular iron depletion stimulates the JNK
and p38 MAPK signaling transduction
pathways, dissociation of ASK1-thioredoxin,
and activation of ASK1,
Journal of Biological Chemistry 2011,
286(17):15413-27
Zhang YM,
Dai BL, A novel
angiogenesis inhibitor impairs lovo cell
survival via targeting against human VEGFR
and its signaling pathway of phosphorylation.
Cell Death and Disease 2012, 3, e406;
doi:10.1038/cddis.2012.145
Zhong D,
Liu X, et al.
LKB1 Is Necessary for Akt-Mediated
Phosphorylation of Proapoptotic Proteins,
Cancer Research 2008;
68:7270-7277.
Zhong D,
Li X, et al.
The Glycolytic
Inhibitor 2-Deoxyglucose Activates Multiple
Prosurvival Pathway Through IGF1R,
Journal of Biological Chemistry 2009; 284:23225-23233.
Additional
publications
Related Products and
Services
Antibody
Array Assay Kit
Protein Extraction Kit
Complete Antibody
Array Assay Service
Antibody
Array Scanning and Image Analysis Service
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