Key Features
Microarray format for high throughput expression
profiling
Suitable samples: cells, frozen or FFPE tissues,
or serum samples
Carefully selected, well-characterized
antibodies
3-D polymer based glass substrates
High specificity and affinity; low background
Excellent uniformity and spot morphology
Each
antibody is printed with replicates
for more reliable and consistent results
Suitable for detection on all fluorescent
microarray scanners compatible with 76x25x1mm
slides
GenePix®
Array List (GAL) files are provided
for convenient and accurate data analysis
Antibody
Array Assay Kit provides
optimized reagents and buffers for processing
the arrays
Applications
Protein Expression Profiling (Qualitative)
Compare profiles of normal, diseased or treated
samples
Identify Candidate Biomarkers
-
Protein Expression
Profiling (Qualitative)
Performance


AKT Phospho-Specific Antibody Array — Lysates
from control and treated H522 cells were labeled
with biotin and detected with Cy3-Streptavidin.
For proteins PTEN(Ser380) and c-Jun(Thr91), the
phosphorylation change between the control
sample and treated sample was 5.59 folds and
4.23 folds respectively.
Components
Antibody Arrays
(2 Slides)
Technical
Information
Antibody
Array User's Guide
Frequently Asked Questions
References
Yalcin A, Clem B,
et al.:
Nuclear
Targeting of 6-Phosphofructo-2-Kinase
(PFKFB3) Increases
Proliferation via Cyclin-dependent Kinases,
Journal of Biological Chemistry, 2009.
Published on May 27, 2009 as Manuscript,
available at:
http://www.jbc.org/cgi/doi/10.1074/jbc.M109.016816
Zhong D,
Liu X, et al.:
LKB1 Is Necessary for Akt-Mediated
Phosphorylation of Proapoptotic Proteins,
Cancer Research 2008, 68: 7270-7277.
Zhong D,
Li X, et al.:
The Glycolytic
Inhibitor 2-Deoxyglucose Activates Multiple
Prosurvival Pathway Through IGF1R,
Journal of Biological Chemistry, 2009.
Published on May 27, 2009 as Manuscript,
available at:
http://www.jbc.org/cgi/doi/10.1074/jbc.M109.005280
Molnar E:
Applications of Protein and Small Molecule
Microarrays, Acta Biologica
Szegediensis 2008, 52(2): 333-356.
Specifications
|
Slide
dimensions: |
76 x 25 x
1 mm |
Spot
diameter: |
260 - 280
um |
|
Barcode
label: |
23 x 9 mm |
Spot-to-spot distance: |
450 - 600
um |
| |
|
|
|
Storage
Conditions
4°C for six months
Related
Products and Services
Antibody
Array Assay Kit
Protein Extraction Kit
Antibody
Array Assay Service
|