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Antibody Microarray

Angiogenesis
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Phospho AKT
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Phospho Insulin Glucose
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Phospho Jak/Stat
Phospho Jak/Stat II
Phospho MAPK
Phospho mTOR
Phospho Neuroscience
Phospho NF-kB
Phospho NF-kB II
Phospho Nuclear Receptors
Phospho p53
Phospho PDGF
Phospho T-Cell Receptors
Phospho TGF-Beta
Phospho VEGF
Phospho TyrosineKinase
Tyrosine Phospho ProArray
Antibody Array Assay Kit

 

Home > Products > Antibody Microarray

Antibody Microarray


Introduction

Antibody Array provides a high-throughput platform for efficient profiling of protein expressions.  Our comprehensive Phospho Explorer Array and Explorer Array allow investigators to examine hundreds of proteins in a single experiment, while the Pathway Antibody Arrays and Phosphorylation Antibody Arrays are designed for researchers to study highly relevant proteins in their specific research fields. 

The antibodies are covalently immobilized on high quality glass surface coated with our proprietary 3-D polymer materials to ensure high binding efficiency and specificity.  Each array includes well-characterized antibodies, positive controls and negative controls. To maximize data reliability, each antibody is printed with six replicates.  The arrays utilize fluorescent detection and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides. 

By utilizing our Complete Antibody Array Assay Service, investigators have the option of sending test samples to experienced scientists at Full Moon BioSystems who will perform antibody array assays in a reliable and efficient manner.

Exploratory Antibody Arrays

Kinase Antibody ArrayNew

Phospho Explorer Antibody Array

Phospho Explorer S1 Antibody Array New

Explorer Antibody Array

Tyrosine Phosphorylation ProArray

Phosphorylation Antibody Arrays

Pathway Antibody Arrays

 


Key Features

  • Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

  • Carefully selected, well-characterized antibodies

  • Each antibody is printed with replicates for reliable and consistent results

  • 3-D polymer based glass substrates

  • High specificity and affinity; low background

  • Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

  • GenePix® Array List (GAL) files are provided for convenient and accurate data analysis

  • Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, coupling and detection


Applications

  • Protein expression profiling (Qualitative)

  • Measure changes in phosphorylation status at specific sites (Phospho Antibody Arrays)

  • Compare profiles of normal, diseased or treated samples

  • Identify candidate biomarkers


Performance

 

AKT Phospho-Specific Antibody Array — Lysates from control and treated H522 cells were labeled with biotin and detected with Cy3-Streptavidin. For proteins PTEN(Ser380) and c-Jun(Thr91), the phosphorylation change between the control sample and treated sample was 5.59 folds and 4.23 folds respectively.


Specifications

Slide dimensions:

76 x 25 x 1 mm

Spot diameter: 260 - 280 um
Barcode label: 23 x 9 mm Spot-to-spot distance: 600 um

Technical Information

Antibody Array User's Guide

Frequently Asked Questions


Components

Antibody arrays (2 slides)


Storage Conditions

4°C for 6 months


Selected References

He H, et al: Sensing the Insulin Signaling Pathway with an Antibody Array, Proteomics Clinical Applications 2009, 3, 1440–1450

Yalcin A, Clem B, et al.: Nuclear Targeting of 6-Phosphofructo-2-Kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases, Journal of Biological Chemistry, 2009 284: 24223-24232

Molnar E: Applications of Protein and Small Molecule Microarrays, Acta Biologica Szegediensis 2008, 52(2): 333-356

Raharijaona M, Le Pennec S, et al. (2009) PGC-1-Related Coactivator Modulates Mitochondrial-Nuclear Crosstalk through Endogenous Nitric Oxide in a Cellular Model of Oncocytic Thyroid Tumours. PLoS ONE 4(11): e7964. doi:10.1371/journal.pone.0007964

Zhong D, Liu X, et al.: LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 68, 7270-7277, September 15, 2008

Zhong D, Li X, et al.: The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathway Through IGF1R, Journal of Biological Chemistry 2009 284: 23225-23233


Related Products and Services

Antibody Array Assay Kit

Protein Extraction Kit

Complete Antibody Array Assay Service

Antibody Array Scanning Service (free of charge)

 

 

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